Clinical Protocols for the Isolation and Expansion of Mesenchymal Stromal Cells
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چکیده
منابع مشابه
Packed Bed Bioreactor for the Isolation and Expansion of Placental-Derived Mesenchymal Stromal Cells
Large numbers of Mesenchymal stem/stromal cells (MSCs) are required for clinical relevant doses to treat a number of diseases. To economically manufacture these MSCs, an automated bioreactor system will be required. Herein we describe the development of a scalable closed-system, packed bed bioreactor suitable for large-scale MSCs expansion. The packed bed was formed from fused polystyrene pelle...
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In recent years, human mesenchymal stem cells (multipotential stromal cells) from bone marrow (hMSCs) have attracted enormous attention owing to their broad therapeutic potential. One of the problems in the overall therapeutic use of hMSCs has been the significant variability in the culture conditions used for their isolation and expansion. Since the seminal publications by Friedenstein and col...
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Mesenchymal stem cells (MSCs) as a kind of adult stem cells possess two properties of long term selfrenewal ability and multilineage differentiation potential into skeletal cell lineages. MSCs were first isolated and described from bone marrow samples. Further investigations have identified several other tissues as alternative sources for these cells. In spite of the clinical importance of MSCs...
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ژورنال
عنوان ژورنال: Transfusion Medicine and Hemotherapy
سال: 2008
ISSN: 1660-3818,1660-3796
DOI: 10.1159/000141567